The large-scale game will see one player competing against a mob of 100 others in a series of trivia challenges for a Microsoft Point prize. The live game is hosted by Chris Cashman, with "Extended Play" sessions open for competition when the show is not airing. 1 vs 100 offers contestants an increasingly high payout as the trivia progresses. The longer the single player answers questions correctly, the more the prize increases, with the "mob" of 100 thinning as they answer incorrectly. The single player can choose to take the prize at any time; if the "one" answers a question incorrectly, the mob splits the purse. The max payout stands at 10,000 Microsoft Points ($125).

The Xbox Live crowd can also compete, with the top three audience members taking home prizes at the end of each show. The top crowd members also raise their chances of being selected to play as a mob member, or as the one.