Priced at $49.99, the collection puts all three games on the same disc, with Metroid Prime 1 and 2 updated to utilize the motion-centric Wii controls of Metroid Prime 3. Unlockable rewards, including music and artwork, have also been added.

Nintendo was clear that "each game maintains its original storyline and settings" as most of the changes relate to controls or graphics, with Retro Studios, which developed the entire first-person adventure trilogy, having handled the compilation. The first Prime entry hit GameCube in 2002, with the second in 2004 and MP3 debuting on Wii in 2007.